动物造模,伤寒沙门菌模型 z-bio 2021-04-07 539

　　(1) Replication method Choose KM mice as the experimental object, and use a gastric applicator weighing 0.1ml/10g to orally oral Salmonella suspension to each mouse. The dilution ratio of the bacterial suspension is 2×1000000CFU/ml.

　　(2) Model characteristics After infection, the activity of all model animals is reduced, and the consumption is also reduced. There were no symptoms of diarrhea or loose stools, and no model animals died. After killing the mouse and performing an autopsy, the intestinal hyperemia and edema, dim and fragile were visible to the naked eye; 6 hours after infection, the small intestine segment showed obvious flatulence and the intestine was severely dilated. After that, the stool is sticky and looks like a colon. The lymph nodes at the edge of the small intestine have enlarged. Histopathological observations under a microscope showed that the villi of the small intestine were lodging, sparse and shedding, and the microvilli were extensively edema and shortened. There is mononuclear cell infiltration in the small intestine tissue, and no intestinal perforation or ulcerative lesions.

　　(3) Comparative Medicine Salmonella typhi is one of the common causes of clinical bacterial enteritis in infants and has a serious impact on children's health. Since its etiology is not yet clear, the innate immune response is the first step for the host to resist Salmonella infection, and is of great significance to the subsequent development and development of inflammation. At present, the study of host resistance to Salmonella typhimurium has attracted widespread attention to early inflammation mechanisms. Therefore, the establishment of this model is of great significance to the study of the molecular immune level of the causes and regulatory mechanisms of inflammation after Salmonella infection, and provides a theoretical basis for clinical anti-inflammatory treatment.