感染性发热模型,动物造模 z-bio 2021-04-07 952

　　(1) Fever model induced by injection of bacterial culture solution 1 that was killed. Model material animal: rabbit; drug: Escherichia coli or paratyphoid broth, glycerin.

　　2. Modeling method: E. coli broth cultured at 30-37°C for 3-4 weeks or B palatinoid meat cultured at 30-37°C for 3-4 days is filtered through a bacterial filter, and the rest is added .. Put the bacterial residue on the filter in a mortar with 1-2 ml of glycerin, grind and autoclave. Take 0.2 ml of the suspension, dilute it with 2 ml of filtrate (broth), and then inject it subcutaneously into the rabbit.

　　3. Modeling principle Injection of killed cells can cause fever in animals.

　　4. Changes after modeling. Within a few hours after the injection, the animal caused a fever, which could last for about 12 hours. (2) Rabbits are injected with typhoid fever-using DPT vaccines for A and B paratyphoid to induce a fever model. 1. Model material: animal: rabbit; drug: typhoid-typhoid DPT vaccine.

　　2. Modeling method Inject 0.5-20 ml of typhoid and paratyphoid A and B triple vaccine into rabbits intravenously, and the vaccine used must be at least 10 billion/ml.

　　3. The principle of simulated infection can cause fever in animals.

　　4. The model changes after 1-2 hours of infusion, and the animal's rectal temperature increases by 1.0-1.5°C within 3-4 hours. (3) Rats injected with Escherichia coli to induce fever 1. Model material animal: rat; drug: Escherichia coli bacterial solution. 2. Modeling method Isolate E. coli strains from urine or ascites, use normal saline to make 0.5ml of bacteria with a concentration of 1,000,000 to 10,000,000, and then intravenously inject them into rats.

　　3. The principle of simulated infection can cause fever in animals.

　　4. Changes after modeling. 3.5-4 hours after the injection, the animal’s exothermic reaction peaked and the body temperature increased by 1.7-2°C. (4) Fever model induced by injection of E. coli or typhoid lipopolysaccharide 1. Modeling material animal: rabbit or rat; drug: E. coli or typhoid lipopolysaccharide.

　　2. Modeling method E. coli or typhoid lipopolysaccharide was injected intravenously into rabbits at a dose of 5-10μg/kg. Alternatively, typhoid lipopolysaccharide is prepared in physiological saline and injected intraperitoneally into rats at a dose of 50 μg/kg.

　　3. The principle of simulated infection can cause fever in animals.

　　4. Changes after modeling may cause fever in animals after injection. (5) Animal fever model of beer yeast

　　1. Modeling materials Animals: rats, weighing 150-200 g, or rabbits; drugs: methyl cellulose, brewer's yeast.

　　2. Modeling method Use 0.5% methyl cellulose or saline, mix brewing yeast (vacuum dried or dried yeast) into 10%, 5%, 1%, 0.2% suspension, and subcutaneous injection dose 10 ml./kg rat is pressed into the back. Alternatively, rabbits were injected intramuscularly with 0.8 ml/kg of 10% beer yeast suspension (prepared with 0.5% methylcellulose).

　　3. The principle of simulated infection can cause fever in animals.

　　4. Changes after modeling The body temperature of rats increased at 3 hours after injection, reached a peak at 6 hours, and increased by 1.2 to 2.2°C. The rabbit's body temperature will rise after the injection, usually by more than 1°C.

　　(6) Fever model of hay rot injection

　　1. Modeling material animal: rabbit; medicine: hay.

　　2. Modeling method: soak about 50 grams of hay in 1 liter of water, and place it in a warm place for 3-4 weeks. The rotten injection solution is then filtered and boiled in the usual way to evaporate to half of the original amount. The filtrate contains metamorphic bacteria, and its metabolites are injected intravenously into rabbits at a rate of 2-3 ml/kg.

　　3. The principle of simulated infection can cause fever in animals.

　　4. After creating the model 1 hour after injection, the animal will have a fever for 8-10 hours.