阿尔茨海默病模型 z-bio 2021-12-07 391

　　1. Modeling materials Animals: healthy Wistar rats, weighing 260-290g, aged 16-17 weeks, males and females; medicine: acetylcholine sinapine (AF64A); equipment: rat brain stereotactic device, micro-motor Dental drill.

　　2. Modeling method After anesthesia, the rat is fixed on the stereotactic fixation device. Cut the skin longitudinally and align the bilateral ventricles at 0.5 mm posterior to the anterior ric, 1.5 mm next to the midline, and 2.5 mm subdural. Gently inject 2.5 μl of freshly prepared 7.5 or 1.5 mol AF64A (about 15 or 30 mol in the whole brain) respectively from one side of the ventricle. The injection speed is about 0.5μl/min. Hold the needle for about 2 to 2 minutes. After 5 minutes, pull out the test tube. Suture the incision. The control group was perfused with the same amount of normal saline.

　　3. Modeling principle Intraventricular injection of the selective cholinergic neurotoxic substance AF64A will damage the cholinergic neurons in the basal forebrain of rats, thereby preparing an AD model.

　　4. Changes after modeling This method can cause cognitive dysfunction in animals, but at the same time it has a profound impact on the motor function of animals and lacks the characteristic pathological changes of AD.

　　5. Precautions Strictly disinfect surgical instruments to prevent surgical infection, surgical trauma should be as small as possible, and strict aseptic operations should be performed. Pay attention to the depth when drilling the skull. If there is a breakthrough, stop immediately. Prevent heavy bleeding. After surgery, the rats can be separated and awakened for 3-5 hours, and then placed in a cage to prevent the first awakened rat from licking or biting the wound of the unconscious rat. In the breeding room, try to maintain the best temperature and humidity for the rats.