动物造模,阿尔茨海默病模型 z-bio 2021-04-07 675

　　1. Modeling materials: Animal: 22-month-old purebred healthy rat, weighing 250-300 g, hermaphrodite, drug: isopentobarbital 2%, equipment: Jiangwan II type brain locator, pen-shaped open cranial drill , Needle electrodes, DC stabilized power supply, current controller, timer, multimeter, stainless steel electrode holder, rat automatic learning and training device.

　　2. Modeling method After abdominal anesthesia with 2% isopentobarbital, the hair was removed from the skull area of the rat, fixed on the Jiangwan II stereotaxic device, and the Koenlng atlas was used for bilateral brain surgery . The basal ganglia are at the base (0.2 mm anterior reg, 25 mm lateral). Cut both sides of the skull with a pen-shaped craniotomy drill to remove the skull section and expose the dura mater. Using a sterile needle electrode, start from the anterior dura mater surface and insert a 7 mm needle at a depth of 2.5 mm perpendicular to the dura mater 0.2 mm in front of the anterior reg. The tip of the needle electrode reaches the basal ganglia of the brain. Use wires to firmly connect the regulated DC power supply, current controller, timer, multimeter and stainless steel electrode holder. One stainless steel electrode clip is fixed to the skin at the incision in the surgical area, and the other (positive electrode) is fixed to the tail end of the needle electrode. Turn on the voltage adjustment switch, start the timer, adjust the output of the current controller, and stimulate the needle electrode with 1mA DC for 30 seconds. Use the same method to damage the basal ganglia on the other side of the rat’s brain. On the fourth day after modeling, the rat automatic learning and training equipment was used to identify and screen learning and memory dysfunction. People with obvious learning and memory impairments are eligible. Select dementia rats suitable for modeling. Experimental Study.

　　3. The modeling principle causes electrical damage to the basal ganglia in the harbor area. Lead to learning and memory dysfunction.

　　4. Changes after modeling The content of acetylcholine in the cerebral cortex was significantly reduced, which impaired the learning and memory abilities of the animals.

　　5. Precautions Strictly disinfect surgical instruments to prevent surgical infection, surgical trauma should be as small as possible, and strict aseptic operations should be performed. Pay attention to the depth when drilling the skull. If there is a breakthrough, stop immediately. Prevent heavy bleeding. After surgery, the rats can be separated and awakened for 3-5 hours, and then placed in a cage to prevent the first awakened rat from licking or biting the wound of the unconscious rat. In the breeding room, try to maintain the best temperature and humidity for the rats.