动物造模,窦房结心律失常动物模型 z-bio 2021-12-09 373

　　[Modeling mechanism] Using mechanical or chemical methods to directly damage the sinus node area, local myocardial ischemia and necrosis, restlessness and conduction disorders, heart rate decline, sinus block, sinus block or junctional stroke and slow syndrome, which cause prolapse Wait.

　　[Modeling method] Use adult male rabbits. 12 hours before the operation, the auricle was anesthetized with pentobarbital sodium (30 mg/kg). After the anesthesia is successful, lie on your back, fix it on the rabbit table, and then connect the electrocardiograph. Open the chest along the left side of the sternum, cut open the bag to expose the heart, use a thin steel wire to loop a half ring with a diameter of about 0.8 cm, wrap a small piece of cotton cloth on it, and then infiltrate 37% formaldehyde. Or before the absolute ethanol penetrates into the root of the rabbit superior vena cava. Stimulate the heart muscle at the junction with the right atrium.

　　[Model Function] The model can quickly change the ECG. The heart rate drops by about 50% and drops to a minimum in about 6-8 minutes. The P wave disappeared after a minute or two. It formed a critical heart rhythm, and a 3-10 ST segment shift (or initial rise and subsequent fall) occurred within a few minutes. The changes in the electrocardiogram are accompanied by a decrease in arterial pressure, which drops to the lowest level in about 8 minutes.

　　[Model evaluation and application] The arrhythmia model using this method has a high success rate, long duration (up to 5 hours), high reproducibility, stable model, etiology and ECG, and its performance is comparable to clinical models similar. .. The extent and extent of damage is controllable. Applicable to the etiology and pharmacodynamic evaluation of human cardiogenic shock.