动物造模,减体积肝移植模型 z-bio 2021-04-12 329

　　With the development of clinically reduced size liver transplantation, rat models of reduced size orthotopic liver transplantation have also emerged. Clinically, it matches the donor liver volume of adult living donor liver transplantation, and the relative and absolute shortage of donor liver volume (donor liver weight is less than 0.8% of the recipient weight ratio) or the liver volume ratio of the transplanted liver recipient GV/SLV is less than 40%. %) The recipient’s hemodynamic changes and unmet metabolic needs can lead to mild liver syndrome. In the rat model, we conducted related studies on reducing three different sizes of liver transplantation.

　　The liver of rats is usually divided into left lobe (left outer lobe), middle lobe (left middle lobe, right middle lobe), right lobe (right upper lobe, right lower lobe), nipple lobe and tail lobe (double tail). ). Long robe)) (Note: The name of the liver lobe varies slightly from book to book. For example, the left middle lobe is sometimes called the left middle lobe). The proportion of each lobe in the liver volume is 32% in the left lobe, 12% in the middle left lobe, 24% in the middle right lobe, 7% in the upper right lobe, 16% and 9% in the lower right lobe in the double tail lobe. The right and middle lobes of the rat were used as donor livers for liver transplantation, and the volume of the liver was reduced to 50%. The survival period is similar to that of whole liver transplantation. The middle lobe of the rat was used as the donor liver for liver transplantation, and the liver volume was reduced to 36%, resulting in a 2-week survival rate of 70%. The middle lobe and right lobe were used as donor livers for liver transplantation and the liver volume was reduced to 24%, and the 11-day survival rate was 30%. The liver function of 24% of rats after right middle lobe transplantation was tested and compared with rats in the whole liver transplantation group. The results showed that ALT and AST were significantly higher than those in the whole liver transplantation group one day after the dose reduction. Large and small liver transplants. ALT and AST subsequently decreased, but were still higher than those in the whole liver transplantation group; from the 3rd day after surgery, serum total bilirubin increased significantly. In addition, color Doppler ultrasound was used to detect portal vein blood flow after liver transplantation. It was found that the postoperative portal vein blood flow rate in the weight-loss group was significantly slower than that of the entire liver transplantation group (5 cm/s vs. 8 cm/s). Prompt the presence of portal hypertension. Therefore, we believe that 36% of liver transplantation will help treat small liver syndrome and can be used as an animal model for small liver syndrome after liver transplantation.