动物造模,双肝移植模型 z-bio 2021-11-23 399

　　In order to reduce the occurrence of small liver syndrome, transplant scientists have made many attempts to expand the size of the transplanted liver. Among them, dual-donor liver transplantation is one of the most effective methods, that is, two donors each take part of the liver and donate it to one liver. Receptor. In order to conduct a systematic study of this procedure, we first established a rat dual-donor liver transplantation model: using the middle liver lobe of one rat and the right middle lobe of the other rat as the donor liver, using the donor’s own liver The inferior inferior vena cava and the left renal vein are used as bypass veins to connect to the portal vein. The superior and inferior hepatic vena cava is connected by a pelvic anastomosis, and the bile ducts are connected by a double stent method, which successfully solves the technical difficulties of double liver transplantation (Figure 4-3). DSA showed that the vena cava, portal vein and bile duct were unobstructed (Figure 4-4). The recipient rats survived for a long time (more than 100 days) after the operation. MRI examination and anatomy of the recipient were performed 7 days and 100 days after the operation. It was found that the transplanted liver was growing well, and there was no necrosis or biliary obstruction. The pathological examination of the liver lobule structure was normal. , No obvious necrosis of liver cells was seen.

　　The establishment and in-depth study of the double liver transplantation model will further reveal the physiological, pathological and immunological changes and mechanisms of double liver transplantation, and provide animal experimental models for clinical work.