去卵巢法骨质疏松,动物造模 z-bio 2021-12-24 289

　　Objective: To replicate the model of human postmenopausal osteoporotic tree shrews through surgical ovariectomy on both sides.

　　Method: In the experiment, 100 female trees were included. Refer to the "Guidelines for the Clinical Diagnosis and Treatment of Primary Osteoporosis" (hereinafter referred to as the "Guidelines") published by the Ministry of Osteoporosis and Bone Mineral Diseases. In 2011, the model evaluation of the Chinese Medical Association: (1) Estimate the peak BMD age of the tree from the natural life span, the time of sexual maturity, the peak bone mineral density (BMD) age, and the life span of the natural tree. And the time of sexual maturity. Randomly select trees with 6, 6, 12, 18, and 24 months old for systematic BMD detection, and compare the differences in BMD in each age group to determine the maximum monthly age of the tree. ② Randomly select monthly peaks. The BMD of 62 BMD ages of the remaining trees sh was tested to determine the average and standard deviation of the BMD peaks of the tree sh. Then they were randomly divided into a model group (n = 32) and a blank group (n = 30). In the model group, two ovaries were surgically removed. In June, September and December, 6 animals were randomly selected for BMD testing. Then select the BMD value that meets the "guidance" diagnostic criteria for pathological examination and confirmation.

　　Results: (1) The peak BMD of the tree sh tested at 6, 12, 18 and 24 months after birth was (0.138±0.012), (0.143±0.010), (0.141±0.009), (0.139±0.009)) g/cm2, yes. There was no significant difference in the BMD peak of tree sh at 1 month old (P\→0.05). (2) The average peak BMD of trees sh tree is (0.143±0.016) g/cm2, ③ the BMD value of each point in the model. This group meets the diagnostic criteria of the "Guide". The number of sh was 0, 5, 4, and 5, respectively. The pathological section of the proximal tibia was compared with the pathological section of a 6-month-old tree sh. Fractures with sparse and widened column spacing; the blank group had the same BMD value at each time point. The number of trees sh used to "guide" the diagnostic criteria is 0, and the pathological section of the proximal tibia and the non-6-month-old tree are abnormal.

　　Conclusion: According to the diagnostic criteria of the "Guide", 6 months after bilateral ovariectomy, a 6-month-old female tree sh can replicate a postmenopausal osteoporosis model.