动物造模,肺动脉高压模型 z-bio 2021-04-13 264

　　Objective: To investigate the expression level and role of RhoA/ROCK signaling pathway in a rat model of left heart disease-related pulmonary hypertension.

　　Method: 20 male SD rats aged 3-4 weeks and weighing 90-100 g were randomly divided into a control group (group C: n = 10) and a pulmonary hypertension group (group H: n = 10). In group H, we fixed and contracted the ascending aorta and established a rat model of pulmonary hypertension associated with left heart disease. The rats in group C received sham operation (fixed the titanium clip on the paravascular mediastinum tissue instead of the ascending aorta, all other operations are the same as in group H), the model is 60. A few days later, each group of rats was tested in order to study hemodynamics (right ventricular systolic pressure, pulmonary artery pressure) and at the expense of rats, PBS was used for in vivo cardiopulmonary lavage. Whiten both lungs and cause left lung tissue. In order to observe the pathological changes of the lung tissue, the pathological section was fixed with 4% paraformaldehyde, and the right lung tissue was frozen for biomolecular detection (Rho kinase mRNA, RhoA mRNA, ET-A receptor mRNA).

　　Result: Compared with group C, pulmonary artery pressure and right ventricular systolic pressure in group H were significantly increased (P\u003c0.01), the pulmonary artery wall was significantly thickened, the pulmonary artery became narrow or narrowed, and even obstruction occurred. The wall enlargement index increased significantly. Large (P\u003c0.01); Compared with group C, the expression level of Rho kinase mRNA in the lung tissue of group H was significantly increased, and the expression levels of RhoA mRNA and ET-A receptor mRNA also increased. Significance and difference are statistically significant (P\u003c0.01).

　　Conclusion: A rat model of left heart disease-related pulmonary hypertension was successfully established through the fixation and contraction of the ascending aorta. Compared with group C, small pulmonary vessel walls in group H were significantly thicker, and the expression of Rho kinase mRNA, RhoA mRNA, and RhoA mRNA and ET-A receptor mRNA in lung tissue were significantly increased, which may be related to signal transduction pathways. In the formation of pulmonary hypertension associated with left heart disease.