动物造模,兔膝关节腔 z-bio 2022-12-29 442

　　Objective: To establish a dual-source CT scan and fluoroscopy to establish a follow-up model of the distribution of sodium hyaluronate (HA) in rabbit knee osteoarthritis, and to observe the influence of different administration sites on the distribution of HA.

　　Method: Use iohexol as a tracer to prepare sodium hyaluronate-iohexol (HA-1) mixtures with iohexol concentrations of 2.5%, 5%, 10%, 20% and 40%. Rabbit knee osteoarthritis, dual-source CT, used for plain scan and three-dimensional reconstruction to screen the best tracer concentration, which can effectively distinguish the drug from the surrounding tissues. Subsequently, 0.5 ml of the HA-1 preparation containing the highest concentration of iohexol was injected into the rabbit knee joint space from the two administration sites below the bone and the joint line. Dual-source CT and X-ray fluoroscopy were used to examine the effect of the drug in knee osteoarthritis. The difference in distribution characteristics confirms the effectiveness of the drug tracking model.

　　Result: The CT value of the HA-I mixture increases with the increase of the concentration of iohexol. When the concentration of iohexol is 2.5%, 5%, 10%, 20% and 40%, the ratio of the average CT value of the knee joint, HA preparation I and bone tissue is 2:7:46, 2:14:44 ., 2:28:44, 2:60:46, 2:98:45, 5%-10% are proven HA-I mixed preparations with iohexol concentration, the drug is most effective from surrounding tissues to obtain ideal The distribution range of the three-dimensional reconstruction effect of the drug. The use of HA-1 preparation with 5% iohexol concentration in CT scan and X-ray follow-up showed that the difference in the administration site was related to the drug distribution. It is mainly distributed in the femoral joint, and HA managed by the joint line is mainly distributed in the tibiofemoral joint.

　　Conclusion: A HA-1 preparation with a concentration of 5% iohexol, combined with CT scan and X-ray fluoroscopy, can effectively distinguish the drug from the surrounding tissues and visually determine the distribution range of the drug in the joint cavity .