动物造模,原发性肝癌 z-bio 2021-04-13 312

　　Objective: To establish a good, fast and stable mouse model of primary liver cancer.

　　Methods: 128 male KM mice were randomly divided into 8 groups: normal diet and normal diet and diethylnitrosamine (DEN) aqueous solution groups containing daily DEN (16.5 mg/kg) aqueous solution; high The dose of DEN sesame oil group (7 times/week, 3 times/week, once/week) orally took DEN (16.5 mg/kg). At the same time, the low-dose DEN sesame oil, nano-DEN and nanocarrier groups received oral DEN (8.25 mg/kg) and nano-DEN or the same amount of nanocarrier oral sesame oil solution every week. Twenty weeks later, the mice were sacrificed, and liver samples were collected, fixed, and then HE stained.

　　Results: (1) Each group of mice, including DEN, had different levels of matte fur color, dullness, slow movement and weight gain. (2) At the same time of 20 weeks, the mice in the high-dose DEN sesame oil group and the DEN aqueous solution group developed tumors at the same time, and the mortality rate of the mice at 1 week/week was very low. 3) The mice in the Nano-DEN group did not die, and HE damaged the liver. The low-dose DEN sesame oil group was serious.

　　Conclusion: Oral administration of DEN dissolved in sesame oil can improve the compliance of mice. Drug therapy and long-term infrequent administration can significantly reduce the mortality rate during the modeling process. In addition, DEN's nano-microemulsion formulation can increase the degree of damage to the liver.