动物造模,早期肺腺癌模型 z-bio 2021-10-28 268

　　Objective: To study the pathological symptoms of early lung adenocarcinoma in mice, focusing on the relationship between tumor growth type and tumor and bronchus.

　　Method: 10 KM mice were given carcinogen nitrosoguanidine (MNNG, concentration 2.0) every week, mg/mL 0.2mg, for 4 weeks. After eating on the 100th day, they were sacrificed, lung lobes were dissected, and tumors were counted visually. After formaldehyde fixation, 100 tumors were randomly selected for paraffin embedding, 3μm sectioning, HE staining, observation and measurement. Observe the histopathological type, size, morphology, edge, growth pattern of the tumor and the relationship with the bronchus under an optical microscope.

　　Result: A total of 187 tumors were observed with naked eyes, and 100 lung adenocarcinomas confirmed by pathology were randomly selected. The tumor size ranged from 0.19 to 1.33 mm, with an average of 0.48 mm. Three growth modes can be seen under the microscope: creep, swelling, and mixing, which are 6, 28, and 68, respectively, and the average size is 0.34, 0.54 mm, and 0.47 mm, respectively. 96 out of 100 tumors are directly related to the bronchus, 13% and 96% of which are related to the ventilating bronchi and ventilating bronchi, respectively. The central and peripheral display rate and the peripheral bronchus of the tumor were 19%, 49%, and 96%, respectively. The average diameters are 67, 91μm and 110μm, respectively. Tumor growth can form leaves (33%) or burrs (35%).

　　Conclusion: The pathological study of the growth mode of early lung adenocarcinoma in mice and the relationship between tumor and bronchus help to better understand the corresponding CT findings of early human lung adenocarcinoma.