动物造模,慢性输卵管炎症模型 z-bio 2022-01-22 546

　　Objective: To establish a model of chronic inflammation of the fallopian tube in New Zealand rabbits using Escherichia coli.

　　How to prepare 3×109/mL mixed bacterial solution (E. coli: Staphylococcus aureus: Streptococcus mixed in a volume of 2:1:1) and 3×108/mL E. coli suspension. Eighteen New Zealand female rabbits were randomly divided into three groups, two of which were in the normal group, eight were in the mixed bacterial solution experimental group, and eight were in the E. coli solution experimental group. The mixture of bacteria and E. coli was injected into two groups of experimental rabbits through vaginal cannula. On the 5th, 10th, 15th and 20th days after the intrauterine injection of the bacterial solution, the formation of fallopian tube inflammation was observed under naked eye observation and optical microscope.

　　Result: The rabbits in the mixed bacteria and E. coli solution showed chronic fallopian tube inflammation on the 15th day of perfusion.

　　End: Both of the two modeling methods have the advantages of being close to the human transmission route, high modeling rate, and easy operation. They are effective methods for establishing animal models of chronic inflammation of the fallopian tubes.