慢性腹泻,动物模型 z-bo 2020-08-05 593

　　Splenasthenic diarrhea (splenasthenic diarrhea) is a TCM syndrome mainly manifested by diarrhea, weight loss, chills, fatigue, etc., including chronic diarrhea, ulcerative colitis, irritable bowel syndrome, and digestive tract in Western medicine. Many diseases such as tumors.

　　[Modeling mechanism] The spleen-deficiency diarrhea model was made with senna leaf and acetic acid. The mechanism is based on the etiology and pathological changes of spleen-deficiency diarrhea and Li Dongyuan’s "bitter cold medicine damages the spleen and stomach", the use of senna leaves to induce diarrhea and acetic local enema It can damage the characteristics of the intestinal mucosa.

　　[Modeling method] Experimental animals: Sprague Dawley rats. Give rats a 20% senna extract at 2ml/kg, once a day [ie 4g/(kg·d)]) for 7 consecutive days. From the 4th day onwards, fasting without water for 32 hours , Inject 0.5ml of 8% acetic acid solution from the anus (depth 8cm), pinch the anus of the rat, and lift it upside down for 20 seconds. Fasting can not help water for 24 hours. MAIN OUTCOME MEASURES: Body weight, loose stool rate (loose stool rate = loose stool number/total stool number), intestinal pathology, serum D-xylose content, serum amylase activity, serum succinate dehydrogenase ratio Activity etc.

　　[Model Features] On the second day of model building, mental fatigue, laziness, arched back appeared, obvious crowding and squinting on the third day, and yellowish coat color, depilation, and pale ear and tail color on the fifth day. After acetic acid enema, bloody stools followed by loose stools and lazy movements.

　　[Model evaluation and application] This animal model conforms to the characteristics of spleen deficiency and diarrhea. The modeled rats showed general symptoms similar to clinical spleen deficiency diarrhea, such as diarrhea, squinting, lazy movement, bunching, dull hair, pale ears , Weight loss, etc., and decreased immune function, spleen index, thymus index, and serum amylase activity. Significant reduction of succinate dehydrogenase. It is consistent with the changes in the syndromes and objective indicators of the syndrome of spleen deficiency and diarrhea clinically, and conforms to the traditional Chinese medicine theory of etiology and pathogenesis. The modeling method can be used for the research of etiology, pathogenesis, pathological changes, the screening of effective drugs for the treatment of spleen deficiency and diarrhea syndrome, and the research of new drug development.