动物造模,肺腺癌A 549绿色荧光裸鼠模型 z-bio 2021-11-12 654

　　OBJECTIVE: To establish a lung adenocarcinoma A549 model inoculated subcutaneously in green fluorescent nude mice, and to study the source of vascular endothelial cells in the process of lung adenocarcinoma tumor angiogenesis.

　　Method: Establish a nude mouse model of A549 lung adenocarcinoma subcutaneously inoculated with GFP, and use immunofluorescence staining for CD31 staining to label tumor blood vessels. Use a fluorescence microscope to observe and photograph frozen sections of tumor tissues. Immunohistochemistry was used to detect the expression of GFP in tumor tissue endothelium.

　　Results: The results of immunofluorescence experiments showed that: GFP protein and CD31 protein can be co-expressed in some interstitial blood vessels; some blood vessels express CD31 protein, but GFP protein. Immunohistochemistry experiment.

　　Conclusion: The cytoplasm of some mesenchymal and endothelial cells in tumor tissues express GFP protein. Conclusion: The endothelial cells that constitute tumor angiogenesis are partly derived from nude mice and partly derived from tumor cells.