动物造模,妊娠期糖尿病小鼠模型 z-bio 2022-01-21 699

　　Objective: To explore a method to establish a stable and reliable mouse model of insulin resistance during pregnancy.

　　Method: 60 SPF 5-week-old KM mice were randomly divided into a high-fat diet group and a normal diet group. The high-fat diet group was fed a high-fat diet for 4 weeks and then placed in a cage. The ratio of male to female is 1: 1. Vaginal suppositories are placed in the vagina on the first day of pregnancy. After a successful pregnancy, intraperitoneal injections were given at 30 mg/kg intervals for 24 hours for a total of 3 injections. The control group was injected with the same amount of citrate buffer (0.1 mol/L, pH = 4.2). On days 3, 7, 14 and 19 after successful modeling, random blood glucose levels, body weight, 24-hour body fluids and food intake were recorded in the mice. ELISA detects the levels of INS, ADP, LEP and CRP factors in serum.

　　Result: After successful modeling, pregnant rats showed obvious symptoms of increased water intake, food intake and urine intake. Compared with the control group, water and food intake are significantly different (P11.1 mmol/L, which is significantly higher than the control group GDM group INS (1.50±0.25) Mu/L, ADP (0.65±) 0.13) μg/L , LEP (1.60±0.12) μg/L, CRP (37.54±4.70) μg/L, the difference is very significant compared with the control group (P\u003c0.01). The blood glucose of the mice returned to normal after delivery.

　　Conclusion: The combination of high-fat diet and low-dose STZ multiple induction method is a GDM model, which has the characteristics of pathological insulin resistance in human gestational diabetes and is very suitable.