动物造模,糖尿病大鼠 z-bio 2021-04-13 330

　　OBJECTIVE: After 9 weeks of modeling with streptozotocin, to detect the expression levels of bladder smooth muscle contraction markers and major regulatory gene myocardium protein in diabetic SD rats to understand whether the bladder smooth muscle of diabetic rats has phenotypic changes.

　　Method: 32 8-week-old male SD rats weighing 200-220g were randomly divided into diabetic (DM) group and non-diabetic (NDM) group. After 9 weeks, the bladder tissue was collected and observed for HE and Masson trichrome staining. Changes in bladder tissue, qRT-PCR and Western blotting were used to detect bladder tissue smooth muscle actin (α-SMA), smooth muscle myosin heavy chain contractile smooth muscle markers, and myocardial gene mRNA and protein expression levels.

　　Results: Compared with the NDM group, rats in the DM group were significantly thinner (286.25±71.20 g vs 412.71±102.74 g, P = 0.001), polydipsia, polyuria, and bladder collagen fibrous tissue increased. u003c0.001), the mRNA and protein levels of myocardin, α-SMA and smooth muscle myosin heavy chain were significantly reduced (all P\u003c0.05).

　　Conclusion: The bladder smooth muscle of diabetic rats undergoes phenotypic changes after 9 weeks of modeling, relaxing and contracting the bladder smooth muscle. It can play an important role in the pathological changes of diabetic bladder.