动物造模,脓毒症模型 z-bio 2022-01-13 671

　　Objective: To establish and evaluate a mouse sepsis model, to study the pathogenic mechanism of sepsis, and to provide model animals for the development of anti-inflammatory drugs.

　　Method: Use cerebellar puncture (CLP) to induce sepsis in mice to improve animal survival, postoperative bacterial load, blood routine and blood biochemical indicators, cytokine levels and histopathological changes, and to treat sepsis in mice. The model will be evaluated.

　　Result: The mortality of mice is closely related to the cecal ligation site. 50% of the mice with cecal ligation had a survival rate of about 40% at 12 days, and 75% of the mice died at 4 days after ligation (P\u003c0.01). Compared with the sham operation group, 50% ligated CLP mice increased blood and intraperitoneal bacteria levels, and decreased white blood cells. The difference is significant (P\u003c0.001). The levels of liver transaminase ALT, AST and serum urea nitrogen BUN of CLP mice increased, the difference was statistically significant (P\u003c0.01), and the levels of inflammatory factors IL1α, IL6, IL10, MIP1α, MIP1β, and TNFα increased. 48 hours after the operation, the liver and lungs of the mice showed obvious histopathological damage.

　　Conclusion: The mouse CLP model has typical pathological features of sepsis, which provides a better animal model for subsequent anti-inflammatory drug research.