阿尔茨海默病,动物造模 z-bio 2021-10-07 382

　　Purpose: Tg2576 transgenic mice showed pathological changes similar to Alzheimer's disease (AD). This article dynamically studied the characteristics of the serum metabolites of Tg2576 mice at various stages of AD, and provided an early diagnosis for the clinical basis of AD metabolism.

　　Method: Collect serum samples of Tg2576 mice in the early (6 months) and final (12 months) stages of AD onset, collect the 1 HNMR spectrum of the samples, and use multivariate analysis techniques to collect metabolic characteristics. . Results: The results showed that the serum metabolic characteristics of Tg2576 and C57 mice were significantly different at 6 months and 12 months, respectively, and there were significant metabolic differences between Tg2576 mice at different stages of AD. Compared with C57 mice, in the early stages of AD development, serum lactic acid, inositol, amino acids (leucine, isoleucine, alanine, etc.) levels in Tg2576 mice increased, lipids and choline, The content of phosphatidylcholine/glycerol phosphatidylcholine and betaine is increased. Glycine and glucose content decreased; at the end of the AD attack, serum lactic acid, inositol and alanine content continued to increase, lipid, choline, phosphatidylcholine/glycerophosphatidylcholine, betaine and glycine content decreased. Glutamate and creatine initially showed a downward trend. Comparing the serum bioconverter of early and late AD, it can be found that in advanced disease, serum lactic acid, inositol, and alanine levels increase, and lipid, choline, phosphatidylcholine, and glycerophosphatidylcholine levels decrease. Among these metabolites, lactic acid, lipids, choline, phosphatidylcholine and glycerol phosphatidylcholine have significant changes in the early stages of AD, and are closely related to the severity of AD.

　　Conclusion: The results show that lactic acid in Tg2576 mice is positively correlated with the severity of Alzheimer's disease, while lipid, choline, phosphatidylcholine and glycerophosphatidylcholine are negatively correlated. Progressive change. It may be an important metabolic indicator for early diagnosis of AD.