动物造模,胶质瘤原位移植瘤模型 z-bio 2021-10-13 312

　　Objective: To establish a stable, real-time monitorable model of glioma allogeneic transplantation in nude mice.

　　Methods: U251 glioma cells were infected with lentiviruses containing luciferase-Luc and green fluorescent protein-GFP genes, and cell lines stably expressing GFP-Luc fluorescence were screened by flow cytometry to make the fluorescent cells proliferate and change Migration and invasion capabilities. Through CCK-8 experiment, cell cycle experiment, Transwell tumor migration and invasion experiment; use the mouse real-time imaging system to monitor tumor growth and inoculate the cells into the caudate nucleus of nude mice to evaluate the pathological characteristics and causes of cells in the brain Tumorous, thereby establishing sympathetic nerves, transplanted in glioma model by paraffin section and HE staining, and transplanted in nude mouse brain.

　　Result: We successfully constructed a U251 glioma cell line and animal model that stably express GFP fluorescence and luciferase fluorescence. Lentiviral integration does not change the cell's ability to proliferate, migrate or infiltrate. This model has a medium growth cycle and high tumor incidence. , The tumor grows stably in the skull, and the HE film conforms to the characteristics of human glioma.

　　Conclusion: Compared with traditional cells, dual-fluorescence-labeled glioma cells can be used in experimental studies of glioma animal models; U251-GFP-Luc glioma cells have similar tumor growth and human nude mouse models. Pathological characteristics. Glioma is similar, and tumor growth can be observed in real time, making it an ideal animal model for experimental research on glioma.