动物造模,动脉移植模型 z-bio 2021-12-15 504

　　Purpose: Allograft vascular disease (AV) limits the function and long-term survival of transplanted organs. This is the most pressing global issue in the field of organ transplantation. Traditional vascular suture requires good microsurgical techniques and is difficult to perform. Exploring more convenient and feasible arterial transplantation methods can provide ideas for clinical research on atrial fibrillation.

　　Method: Use an indwelling venous needle to make a self-made vascular cannula, pull the thoracic aorta of the donor rat into the cannula, turn both ends upside down, ligate and fix it to the graft, and then implant the graft. In order to observe the morphology of the transplanted blood vessel, the thickness of the endometrium was measured at 1, 4, and 8 weeks after the recipient’s abdominal aorta was modeled to perform HE staining on the transplanted artery.

　　Result: 38 of the 40 recipient rats survived to the required detection point, their lower limbs and tail moved freely, and their urine and urine were normal. HE staining showed that the endometrium of the transplanted blood vessel had concentric circles. AV type thickening.

　　Conclusion: This method of rat artery transplantation is simple and feasible. Compared with traditional vascular suture, it significantly reduces the occlusion time of the abdominal aorta, reduces the surgical trauma of rats, and improves the success rate.