动物造模,Ⅱ型糖尿病 z-bio 2021-04-14 260

　　Objective: To establish a model of exercise to improve glucose metabolism in type 2 diabetic rats, and to provide a theoretical reference for the establishment of exercise prescriptions for type 2 diabetes.

　　Methods: Forty-five SPF male Wistar rats aged 8 weeks, 32 were randomly selected and fed with a high-sugar and high-fat diet for 7 weeks and injected intraperitoneally with a small dose of streptozotocin (STZ) buffer to establish type 2 diabetes mellitus Rat model. Normal rats and successfully modeled rats were divided into 4 groups: blank control group (C group), simple exercise group (CE group), diabetes control group (DM group), and diabetic exercise group (DME group). The exercise group used an improved Ploug training program, 60 min/d, 6 days a week, a total of 8 weeks of training. After 7 weeks of high-fat diet, blood was collected from the tail vein to determine fasting blood glucose and serum insulin. After modeling, blood was collected from the tail vein at baseline and 8 weeks after exercise to determine fasting blood glucose (FBG), and blood was collected after 8 weeks of exercise to determine serum insulin. (FINS), and calculate the insulin resistance index (HOMA-IR).

　　Results: ①After 7 weeks of high-sugar and high-fat feeding, the content of FBG, FINS and HOMA-IR in the high-fat group was significantly higher than that of the normal group. ②After 8 weeks of exercise intervention, FINS levels in DM and DME groups were significantly lower than those in C and CE groups, FBG and HOMA-IR were significantly higher than in C and CE groups; FINS levels in DME group were significantly higher than those in DM group, FBG and HOMA- IR was significantly lower than the DM group. The body weight of the DM group and the DME group was significantly lower than that of the C group and the CE group; there was no significant difference in body weight between the DME group and the DM group, and the CE group and the C group.

　　Conclusion: ①The 7-week high-sugar and high-fat feeding combined with one-time intraperitoneal injection of STZ (30 mg/kg) successfully established the type Ⅱ diabetes rat model; ② The swimming exercise pattern of 60 min/d for 8 weeks can improve the glycemia of type Ⅱ diabetes rats Metabolism is an ideal animal model for studying the mechanism of exercise prevention and improvement of diabetes.