动物造模,慢性非细菌性前列腺炎动物模型 z-bio 2022-01-17 516

　　Objective: To establish an animal model of chemical chronic non-bacterial prostatitis, and to provide a reliable model animal and evaluation method for the pathogenesis and drug research of CNP.

　　Methods: SD male rats were randomly divided into control group and model A, B, and C groups. Model A, B, and C were injected with 1% sterile carrageenan into the left and right ventral lobes of the prostate. 50, 100 μL; each in the control group was injected with 50 μL sterile saline solution. 7-day post-treatment, from anatomical point of view, prostate index, white blood cell detection, pathological section, tumor necrosis factor-α (TNF-α), nuclear factor-κB (NF-κB), IκB kinase (IKKα), phosphorylated IKB -α (p-IKB-α) and cyclooxygenase-2 (COX-2) protein expression affect 5 aspects to be analyzed.

　　Results: Compared with the sham operation group, the softness of the prostate tissue in the model A, B, and C groups decreased, the elasticity was weakened, and the adhesion with the surrounding tissues occurred; the prostate index and the total number of white blood cells increased significantly (P<0.01), A, B, C The increase rate of prostate index in group was 21.1%, 61.7%, and 72.7%; the increase rate of total number of white blood cells was 75.0%, 103.6%, and 114.8%; pathological results showed that the prostate interstitial swelling in group A was less, and the interstitial swelling in group B and C was less. Significant swelling of the prostate, obvious atrophy of the prostate in group C, and degeneration and necrosis of some glands; the expression levels of NF-κB, IKKα, p-IKB-α, TNF-α and COX-2 in the prostate tissues of groups A, B and C showed varying degrees Increased, the NF-κB inflammation channel is activated.

　　Conclusion: Injecting 20, 50, 100 μL of 1% carrageenan on the left and right ventral lobes of rat prostate can induce different degrees of prostatic inflammation. However, if the dose of 20 μL is too small, the inflammatory response is weak and the operation error is large; The inflammatory response of 100 μL is too strong, and some animals die; the animal model of prostatitis caused by the dose of 50 μL is the most successful, and it can obviously activate the NF-κB inflammatory signal pathway.