动物造模,卵巢早衰动物模型 z-bio 2021-11-05 353

　　Objective: To compare different injection methods to establish an animal model of cyclophosphamide-induced primary ovarian insufficiency, find the best modeling method, and provide a more suitable experimental model for further research on early ovarian insufficiency.

　　Method: 21 rabbits were randomly divided into 4 groups, 3 normal control group, no treatment (group A), 6 model group, cyclophosphamide solution 50 mg/kg injection for 1 day (group). The second group of 6 people, 50 mg/kg. Continuous injection for 2 days (group C); 6 rats in the 3 groups of models were injected with 50 mg/kg on the first day, and then at 8 mg/kg for 14 consecutive days (group D)? Change the body weight of the rabbit within 14 days after injection and consider each group's ovary and weight, ovarian index analysis, HE staining to observe the shape of follicles, counting the number of follicles at all levels under an optical microscope, and TUNEL method to observe and analyze the cell population; used to analyze serum The ELISA method for estradiol (E2) changes?

　　Result: Weight: B? There was no significant change in body weight in group C (P\→0.05); growth restriction of rabbits in group D (P0.017)? In the three groups A, B and C, there was no significant difference in the abnormal rate of secondary antral follicles (P\→0.05). Apoptosis rate: Apoptosis mainly occurs in granuloma cells of follicles. Group B and C were significantly higher than group A (P\u003c0.05), the apoptosis rate of group C was higher than group B (P\u003c0.05) 0.05, serum E2 value: E2 secretion in group B was on the 7th day on the 7th day. The days gradually decreased, and the E2 secretion on the 7th day was significantly higher than that on the 35th day (P\u003c0.05). The E2 value of group C continued to decrease, and the 0th day was significantly higher than the 35th day (P\u003c 0.05)?

　　Conclusion: Is 50 mg/kg continuous injection for 2 days the best way to establish a rabbit model of early ovarian failure induced by cyclophosphamide?