动物造模,全子宫切除模型 z-bio 2021-12-30 431

　　Objective: How to establish a BALB/c mouse total hysterectomy experimental animal model, and investigate and analyze the characteristics and formation mechanism of the model.

　　Method: Divided into blank control group, sham operation group and model group. The blank control group received no treatment. Reproduction; the model group undergoes total hysterectomy through the abdomen; does the sham operation group just cut the skin to expose the uterus? They were reared regularly after the operation, and the weight changes of each group of mice were dynamically observed, and the peripheral blood estradiol content and ovarian tissue were detected at the end of the fourth week.

　　Result: All experimental mice in each group survived the operation and were completely cured, with good general symptoms, and no obvious inflammation in the incision. Is there a significant difference between the preoperative body weight of each group of experimental mice and the body weight 2 weeks after surgery? In the 3rd and 4th weeks, the weight of the mice in the model group exceeded that of the control group and the dummy. Surgery group (P\u003c0.05); the peripheral blood estradiol content of the model group was significantly lower than the control group and the sham operation group (P\u003c0.01); the blank control group and the sham operation group of the ovarian histology model group Show abnormal histological changes, such as irregular follicular morphology, loose granular cells and apoptosis?

　　Conclusion: This experimental animal model can successfully simulate the clinic. Are the postoperative ovarian reduction and other clinical symptoms ideal? An animal model for studying complications after hysterectomy and screening drugs?