动物造模,心肌缺血再灌注损伤模型 z-bio 2021-04-15 292

　　Objective: To improve the method of establishing a rat model of acute myocardial ischemia-reperfusion injury (MI/RI), to conduct a comprehensive evaluation, and to lay a good model foundation for future MI/RI research.

　　Methods: 36 clean Wistar rats were randomly divided into 3 groups: normal group (normal group), left anterior descending artery (LAD) without intervention, sham operation group (sham group), LAD with only threading and no ligation: ischemia In the reperfusion group (MI/RI group), LAD was ligated for 30 minutes and reperfused for 120 minutes. After the rats were anesthetized, the electrocardiogram was recorded continuously, a ventilator was used for tracheotomy, and the rat's LAD was involved in the thoracotomy. Improved electrocardiogram, measurement of myocardial enzyme content, area of myocardial infarction and histopathological examination of myocardium. Comprehensive evaluation of the model.

　　Results: The ST-T dynamic changes of the ECG in the MI/RI group were obvious; the creatine kinase isoenzyme (CK? MB) level of the MI/RI group increased significantly after 2 hours; compared with this group, the MI/RI group The levels of creatine kinase isoenzymes were significantly increased. The infarct size increased significantly, the cardiomyocytes in the MI/RI group were degeneration and necrosis, and the inflammatory cells infiltrated extensively.

　　Conclusion: The improved modeling method not only reduces the difficulty of surgery, but also successfully establishes a rat MI/RI model, laying a good model foundation for future MI/RI research.