动物造模,化疗性肠黏膜炎模型 z-bio 2021-11-29 294

　　Purpose: To study the 5-fluorouracil-induced animal model of chemotherapy-induced intestinal mucositis in mice.

　　Methods: Different doses of 5-FU were administered intraperitoneally or continuously for 5 days to mice. The body weight and diarrhea of the mice were observed daily. At 72 hours or 24 hours after the last administration, the peripheral blood and blood conditions of the mice were observed. Histopathological changes in the small intestine.

　　Results: Compared with the normal group, after a single or continuous 5-day administration of 5-FU, mice in each dose group experienced varying degrees of diarrhea and weight loss, and the levels of white blood cells and platelets in the peripheral blood were significantly reduced (P<0.05 or P < 0.01), the single-dose 400 mg/kg group and the continuous 50,100 mg/kg group showed obvious pathological features of intestinal mucositis. The 100 mg/kg group was overdose and the mortality rate reached 100%.

　　Conclusion: Single or continuous 5-day administration of 5-FU has a dose-dependent effect on intestinal mucositis in mice. 400 mg/kg is the appropriate dose for a single dose, and 50 mg/kg for a continuous 5-day dose. The right dose.