动物造模,糖尿病大鼠模型 z-bio 2021-04-15 270

　　Objective: To observe the changes in the structure and abundance of the intestinal flora in a diabetic rat model induced by streptozotocin.

　　Method: 25 male SD rats were randomly divided into control group (C) 10 rats and model group (M) 15 rats. The model group was intraperitoneally injected with 3% STZ at a dose of 30 mg/(kg·bw) for 5 consecutive days. After the model is formed, measure the weight, blood sugar and other indicators every month. When the model was established for 4 weeks and 12 weeks, fresh rectal feces from each group of rats were taken at the same time. The 16S rDNA-V3 region of bacteria in fecal samples was sequenced on the Illumina high-throughput sequencing platform, and the structure and abundance of the intestinal flora were quantitatively analyzed.

　　Result: There is no significant difference in the number of optimized sequences between the model group and the control group (P>0.05). The relative abundance index and diversity index of the intestinal flora in the model group were lower than that of the control group (P<0.05), and the Simpson index was higher than that of the control group (P<0.05). At the phylum level, the relative abundance of Proteobacteria, Cyanobacteria, Soft-walled Bacteria, TM7, Actinomycetes decreased (P <0.05). At the genus level, the relative abundance of Lactobacillus in the model group decreased (P <0.05); the relative abundance of Bacteroides increased (P <0.05). After 12 weeks of modeling, the relative abundance of Lactobacillus, Bacteroides, and Rumenococcus in the model group increased (P <0.05); the relative abundance of Bifidobacterium decreased (P <0.05).

　　Conclusion: The abundance and diversity of the intestinal flora of diabetic animals in SD rats induced by STZ decreased, which provides a reference for the study of the relationship between diabetes and intestinal flora.