动物造模,胃炎癌转化模型 z-bio 2022-01-25 289

　　Objective: How to establish a rat gastritis and gastric cancer transformation model and explore new serological diagnostic indicators for early gastric cancer?

　　Method: N-methyl? '-Nitro? How to use N'-nitrosoguanidine drinking water (MNNG) in combination with high-salt feeding: inducing a rat gastritis cancer transformation model? MRI and histopathology to observe the deterioration process of rat stomach? qRT? At different time points (modeling 0, 124, 36, 48 weeks) -17 expression levels of 5p and miR-374-5p? PCR detection of rat serum miR.

　　Result: MNNG drinking water and high-salt diet successfully induced the transformation of gastritis in rats, and experienced atrophic gastritis (modeling for 24 weeks) and dysplasia (modeling). The levels of miR-17-5p and miR-374-5p in serum are positively correlated with the degree of gastric degeneration in rats. Compared with the normal control group, the expression level of miR-17 -5p increased by about 8 times (t = 4.1P\u003c0.001) and 10 times (t = 5.3P\u003c0.001); compared with the normal control group , An increase of 48 times. One week later, the expression level of miR-374-5p increased by about 6 times (t = 3.60.001\u003cP\u003c0.002). )?

　　Conclusion: The rat gastritis transformation model established by MNNG drinking water and high-salt feed supplies provides an excellent animal model for studying the etiology of gastric cancer. Are serum miR-17-5p and miR-374-5p potential indicators for early non-invasive diagnosis of gastric cancer?