动物造模,溃疡性结肠炎模型 z-bio 2021-12-21 412

　　OBJECTIVE: To investigate the molecular significance of papaverine's regulation of Chinese herbal medicine regression theory on respiratory tract and inflammatory bowel disease. Analyze the changes of differentially expressed genes in the two animal models of disease after treatment, and clarify the molecular mechanism of lung-intestinal correlation

　　Method: The allergic asthma model using the ovalbumin OVA spray method is compared with the ulcerative colitis model established through the joint immunotherapy. Use normal rats as controls. After administration of papaverine, reverse transcription polymerase chain used RTPCR to detect the mRNA expression levels of differentially expressed genes in the lung and intestine tissues of the two groups of model rats

　　Result: Papavelin in rats can significantly improve the pathological changes of lung and kidney colon tissue. Sequencing is analyzed by KEGG annotation. Three important differences. The next gene to be expressed is C￣C motif Chemokine 11 Carbonate dehydratase 1 Nuclear hormone receptor subfamily D member 1T￣ PCR results are basically consistent with the transcriptome sequencing results, which reduces The expression level of Ccl11 mRNA in the lung The two groups of papaverin up-regulated the colon and colon tissues of model rats (P\u003c005) and increased the expression levels of Ca1 and Nr1d1 mRNA (P\u003c005P\u003c005)

　　Conclusion: Do rats suffer from respiratory tract and inflammatory bowel disease? The intestinal tissue poppy with the same differentially expressed genes can significantly regulate the level of differentially expressed genes between the lung and the large intestine. This may be one of the molecular mechanisms of papaya return.