动物造模,鹌鹑痛风模型 z-bio 2021-04-16 266

　　OBJECTIVE: By simulating clinical high-protein and high-calcium diets to induce quail gout in Difalco, to preliminarily explore the method of establishing a quail gout model, so as to provide basis for the research on the pathogenesis of gout and the screening of prevention and treatment drugs.

　　Method: 36 30-day-old male healthy Defaque quails were selected and randomly divided into 3 groups based on body weight and blood uric acid level, namely the control group, the model I group and the model II group, each with 12. The control group quail feeding basis Diet, the model group was fed a high-protein and high-calcium diet (the crude protein content of the diet was 29.5%, the calcium content was 7.8%), the control group and the model I group had free drinking water, and the model II group was restricted to drinking water for 4 hours a day, and the experiment lasted 50 days. Detect quail serum, feces, urine and uric acid levels, at the same time, detect renal function and observe histopathological sections.

　　Results: High-protein and high-calcium diets can significantly increase quail serum uric acid levels; significantly reduce fecal and urine uric acid levels; increase serum creatinine and urea nitrogen levels; lead to significant urate deposition in quail kidneys. Combination of high-protein and high-calcium diets Restriction of drinking water can aggravate the increase in blood uric acid levels and kidney urate deposition, causing some quails to develop joint swelling, deformation and other symptoms of gout.

　　Conclusion: Feeding high-protein and high-calcium diets and restricting the amount of water can establish a gout quail model, and its pathogenesis is more in line with the clinical etiology, pathogenesis and clinical manifestations of gout, providing reference for the basic and drug development research of gout.