动物造模,支气管哮喘模型 z-bio 2022-01-17 307

　　Objective: To study the effects of different doses of ovalbumin (OVA) on serum IgE, IL-4, IFNγ, bronchoalveolar lavage fluid (BALF) and eosinophils (EOS) in a BALB/c mouse bronchial asthma model. First determine the more appropriate OVA model dose?

　　Method: The BALB/c mice were randomly divided into normal group, model 1 group (OVA20μg), model 2 group (OVA50μg), model 3 group (OVA100μg) and dexamethasone. Acetate (OVA 100μg)? Have you established a bronchial asthma model by intraperitoneal injection and sensitization of the OVA dose of mice in other groups except the normal group and stimulation with 1% OVA spray? ELISA to detect serum IgE? IL-4? IFN-γ content, Wright's staining can calculate the EOS number of BALF, HE staining can observe the pathological changes of lung tissue?

　　Result: Compared with the normal group, the serum IgE of each group of mice is higher? The EOS of IL-4IFN-γ and BALF was significantly increased (P\u003c0.05), IgE? IL-4? In IFN-γ, inflammatory cell infiltration occurs in lung tissue, accompanied by bronchial epithelial cell degeneration, bronchial smooth muscle cell proliferation and other pathological changes. Are the lung tissue content and pathological changes of the mice in the OVA 50μg group more obvious?

　　Conclusion: Three doses of OVA can successfully induce asthma in BALB/c mice. Generally, the asthma model induced by 50 μg OVA is more pronounced. Is it recommended as a suitable model dose for the .BALB/c mouse asthma model?