动物造模,结肠癌肝转移裸鼠模型 z-bio 2021-04-16 389

　　Objective: How to establish a colon cancer liver metastasis model with high metastasis rate? Is the operation simple? Reliable results of experimental studies on the prevention and treatment of colon cancer metastasis?

　　Method: 15 Balb/c nude mice were divided into three groups (group A (group B, group C), and 5 Balb/c mice were divided into group D. The colon cell concentration was constructed by spleen transplantation and preservation of the spleen 2.5 x 107/mL 0.2 mL HCT116CT26 cell suspension model cancer liver metastasis model, success rate and liver metastasis size. Do you want to compare the four groups of animal models? Number and intraperitoneal metastasis?

　　Results: The success rate of the nude mouse model in group A was 100% (5/5). All the liver and spleen formed tumors. Liver metastases were scattered and mostly distributed in the right lobe of the spleen. The average survival time was (26.6±3.4) days. The success rate of nude mice in group B is 40% (2). 5) The metastatic tumors were scattered on the surface of the spleen and the volume was larger than that of group A. The average survival time of nude mice in group C was (36.8±4.2) days; the success rate of adult mice was 100% (5/5/). 5) The liver and spleen are tumors, and the number of liver metastases is relatively high. Many metastases fused into clusters, occupying the entire right lobe of the liver, with an average survival time of (20.2±2.6) days; no metastases were found in the liver of group D; abdominal metastases appeared in three groups of nude mice (groups A2 and C3) , No colon, lung, brain or kidney metastasis. Does the histocytology of the three groups of liver metastases meet the characteristics of adenocarcinoma?

　　Conclusion: Preservation of the spleen can increase the success rate of the model. Can the methods and processes that are effective for the treatment of the human colon be simulated by the transfer of blood-mediated cancer cells to the spleen?