动物造模,结核小鼠感染模型 z-bio 2021-12-09 423

　　Objective: To use two cultures of Mycobacterium tuberculosis (BACTEC MGIT960 culture and Roche (L? Wenstein-Jensen, LJ) culture) to simultaneously verify the therapeutic effect of rifampicin (RIF) in a mouse model of tuberculosis infection. What is the application value of using the BACTEC MGIT960 culture system in a mouse model of bacterial infection?

　　Method: The experiment was divided into BACTEC MGIT960 culture group and LJ culture group, and 36 C57BL/6 women were infected with 1.0×106 CFU/tail vein injection respectively. In mLH37Rv bacterial solution mice, 18 mice were treated with rifampicin 4 weeks after infection, 18 mice were injected with the same amount of phosphate buffered saline (PBS) as the control group, 36 mice were injected, BACTEC MGIT960 rapid culture and Roche culture . Lung, spleen and spleen. Do you want to culture liver tissue homogenate?

　　Results: When using the BACTECMGIT960 culture method, the positive culture time of liver, lung and spleen tissues in the RIF treatment group is (187.11±10.20) h? (187.11±10.20) hours? (347.22±12.70) hours? (276.39)±13.09)h, the control group is (142.50±11.70)h? (251.67±16.63) hours? (230.28±7.22)h, the difference between groups was significant (P\u003c0.001); Roche culture, RIF treatment group liver, lung, spleen tissue bacteria amount (5.15±0.15) log10CFU? (3.30±0.23) log10CFU? (3.40±0.25) log10CFU, control group (5.90±0.25) log10CFU? (3.88±0.31)) log10CFU? (4.15±0.30) log10CFU, is there a significant difference between the groups (P\u003c0.001)?

　　Conclusion: Using BACTEC MGIT960 culture and Roche culture to detect the number of bacteria, the culture results are the same, but the former cuts the time to detect positive results by an average of half and can be identified, which demonstrates the BACTEC MGIT960 culture method to a greater extent It has more advantages in detection. Can it be used for the detection of bacterial load in animal models of tuberculosis infection?