动物造模,胰岛素抵抗动脉粥样硬化模型 z-bio 2022-04-20 449

　　Objective: To investigate the phenotypic differences and pathological mechanisms of insulin resistance atherosclerosis models in white-haired black rabbits and Japanese big-eared white rabbits.

　　Method: Take 12 WHBE rabbits and 12 JW rabbits, and divide them into normal control group (NC) and high-fat and high-sugar diet (HF) groups, each with 6 rabbits. IR-AS model was induced with HF diet for 12 weeks. End of model building After that, blood was taken to determine blood lipids, superoxide dismutase (SOD) and malondialdehyde (MDA) levels; glucose tolerance test was performed to calculate the area under the curve of blood glucose and insulin; liver microsomal triglyceride transfer protein (MTTP) was detected. Expression of nuclear factor E2 (Nrf2) and SOD1 genes, and observe the pathological changes of HE staining of fat and aortic blood vessels, and the expression of CD68 in blood vessels.

　　Results: Compared with the NC group, the HF group was obese, blood lipids increased, glucose intolerance, hyperinsulinemia and insulin resistance index (HOMA-IR) increased significantly, plasma and liver SOD activity decreased and MDA content increased, liver MTTP and Nrf2 gene expression increased and SOD1 gene expression decreased, vascular lipid deposition and AS and vascular CD68 expression were significantly increased; compared with the JWHF group, the area under the TG, LDL-C, HOMA-IR, glucose tolerance curve in the WBHF group ( U_ GLU), MDA content, fat diameter, liver SOD1 gene expression, AS lesion degree and vascular CD68 expression are significantly different.

　　Conclusion: High-fat and high-sugar diet can induce the formation of IR-AS in rabbits, showing lipid metabolism disorders, inflammation and AS lesions. However, the degree of lesions in WHBE rabbits is significantly more severe than that in JW rabbits, which may be related to the lipid metabolism of these two strains of rabbits. It is related to the difference in oxidative stress.