动物造模,人肝癌裸小鼠原位癌模 z-bio 2021-04-16 412

　　Objective: How to select a human hepatocarcinoma cell line (HCCLM3-Luc) stably expressing a luciferase reporter gene and construct a nude mouse model of the human hepatocarcinoma cell line in situ?

　　Method: In vivo imager quantitatively analyzes the expression of HCCLM3-Luc fluorescence intensity of various cell numbers, the detection of luciferase activity per cell number, the number of photons produced by HCCLM3-Luc luciferase, and the number of tumor cells. Whether to investigate between them The linear correlation? Inoculate HCCLM3-insituLuc tumor mass tissue with liver leaves and inject HCCLM3-Luc cell suspension into tail vein to establish human liver cancer nude mouse model in situ; the key factor for comparison of human liver cancer cell lines in nude mice is to establish the model and optimize the model construction. The feasibility of the method? Results: The number of HCCLM3-Luc cells was positively correlated (correlation coefficient R2 = 0.9989, linear equation: Y = 1155.8X +1×106), and the average ROI value was about 140 photons/sec/sec. cell? Is the incidence of tumor in situ in the liver lobe orthotopic vaccination group [(82.5±7.2)%] significantly higher than that of the tail vein injection [[34.1±13.2]%]?

　　Conclusion: This project is a nude mouse model of in situ bioluminescence human liver cancer. Can small animal imagers be used to monitor tumor growth in real time?