动物造模,测定肺动脉高压小鼠模型 z-bio 2021-04-19 370

　　Objective: To establish a rapid and effective method for measuring right ventricular pressure in mice, which can be easily operated without the use of a ventilator, and to explore measurement techniques.

　　Method: 23 C57BL/6J mice (8 weeks old) were randomly divided into hypoxia group (11) and control group (12). The hypoxic group was kept in a hypoxic chamber (10% O2) for 4 weeks. In the pulmonary hypertension model, the control group was kept in normal air, and all other conditions were the same. After the model was created, a 0.6 x 25 mm disposable infusion set needle was used to puncture the right ventricle directly through the intercostal muscle with the mouse's chest cavity closed, and the right ventricular systolic pressure (RVSP) was measured in multiples. -Turn on the physiological machine and chest to check the puncture point. Locate and separate the heart, weigh the right ventricle (right ventricle, RV), left ventricle, and ventricular septum (left ventricle + septum, LV + S). Calculate the right ventricular hypertrophy index (right ventricular hypertrophy index, RVHI = V / (LV + S) × 100%), take the left lung as a pathological section, and use hematoxylin and eosin (HE) and α-smooth muscle actin ( The structure was observed by staining with α-SMA. Pulmonary artery changes.

　　Result: The right ventricle was directly punctured by the trans rib to obtain the right ventricular pressure curve. The whole process is completed in 3-4 minutes. The success rate of VSP measurement was 95.6% (one failure). The RVSP and RVHI of the hypoxia group were (30.48±2.95) mmHg and (35.31±3.78)%, respectively, which were significantly higher than (17.50±3.40) mmHg and (23.14±4.29)%. Control group (all P\u003c0.05). The pulmonary arteries of mice in the hypoxic group were significantly thickened. Proliferation of smooth muscle in the medium.

　　Conclusion: The method of measuring the right ventricular pressure curve of mice by intercostal muscle puncture is maintained. The breasts of animals are tight and do not need to be ventilated with a ventilator. Simple and fast, you can quickly assess the hemodynamic status of the right ventricle of mice.