动物造模,小鼠肺癌模型 z-bio 2021-04-19 255

　　Objective: To study the expression pattern of TET family proteins in mouse lung cancer models.

　　Method: Divide mice into 3 groups, each with 10 mice. Set up as control group (Con), urethane induction group (Ure) and hydroxyurea administration group (Ure-HU). Ure and Ure-HU groups were administered 800 mg/kg uranium solution for 10 weeks to induce the establishment of lung cancer models in mice. After 10 weeks, HU drug (500 mg/kg) was administered to the Ure-HU group for 21 days. Observe the number of lung nodules. The TET expression patterns in the three groups of mice were analyzed by qPCR and Western blotting.

　　Result: Compared with the Con group, the weight loss of Ure and Ure-HU groups was significant. Morphological observation showed that both Ure group and Ure-HU group had lung tissue damage. The qPCR results showed that the expression of TET in the Ure group was significantly reduced (P\u003c0.05). After HU treatment, the expression of TET1 and TET2 in the Ure-HU group increased (P\u003c0.05). Western blot and qPCR results are consistent.

　　Conclusion: In the mouse lung cancer model induced by uracil, the expression pattern of TETs protein is abnormal, indicating that TET1 and TET2 play an important role in the development of lung cancer.