动物造模,C57BL6小鼠肝癌模型,肝癌模型 z-bio 2021-04-19 360

　　Objective: To establish a chemically induced C57BL/6 mouse liver cancer model and simulate the occurrence and development of liver cancer to explore the mechanism of liver cancer.

　　Method: Select 110 C57BL/6 mice and randomly divide them into a control group (30) and a model group (80). The mice in the control group were not treated, and the model group established a liver cancer model by intraperitoneal injection of CCl4 and DEN. The mice were continuously weighed, and the mice were dissected at different times to observe the liver morphology, and to measure the levels of liver function-related enzymes in the venous blood. And observe the pathological changes of liver tissue.

　　Results: Compared with the control group, the mice in the model group gradually lost weight, had abnormal liver morphology, and elevated serum levels of liver function-related enzymes ALT and AST, which were also obvious in liver cancer, and histopathological changes were observed.

　　Conclusion: The combination of diethylnitrosamine (DEN) and carbon tetrachloride (CCl4) may induce liver cancer in C57BL/6 mice. This is a reliable animal for studying the molecular mechanism of liver cancer and provides a model.