动物造模,非开胸式主动脉弓狭窄心衰模型 z-bio 2021-04-19 273

　　Objective: To establish a stable and reliable mouse heart failure model of non-thoracic aortic arch stenosis, and to analyze and observe the pathological process of heart failure in mice. Established by non-thoracic TAC surgery

　　Method: C57BL/6J mouse heart failure model, and evaluate the heart function and the degree of heart failure through echocardiography, left ventricular mass index and histopathological examination.

　　Result: The left ventricular mass index gradually increased over time. Compared with the sham operation group, there are very significant differences in the 4th, 8th and 12th weeks after the operation (P\u003c0.01). On echocardiography, compared with the sham operation group (left), the left ventricular anterior wall thickness, left ventricular corrected weight, heart rate and cardiac output increased significantly after 4 weeks of TAC (P\u003c0.01). The weight of the left ventricle increased significantly 8 weeks after the operation. At 12 weeks after TAC, the left ventricular end diastolic diameter (LVID) was D), the end diastolic volume of the left ventricle increased significantly (P\u003c0.01), and the excretion rate and short-axis velocity decreased significantly (P\u003c0.01). Diastolic diameter, left ventricular end-diastolic volume and left ventricular end-systolic volume increased significantly (P\u003c0.01), excretion rate and short axis velocity were further reduced (P\u003c0.01), pathological examination showed abnormal or varying degrees of myocardial fibers Of lesions.

　　Conclusion: The experimental results show that the establishment of a mouse heart failure model by non-thoracic TAC is stable and feasible, and can simulate the pathophysiological process from left ventricular hypertrophy to heart failure caused by artificial pressure overload.