动物造模,免疫力低下小鼠动物模型 z-bio 2021-04-19 456

　　Objective: To quickly establish a low-immunity KM mouse model, and determine the establishment of an animal model in a simple and rapid way.

　　Method: 100 mg/kg cyclophosphamide in oral gastrointestinal nutrition solution, hydrocortisone in oral gastrointestinal nutrition solution 80 mg/kg, 60% ethanol solution in oral gastrointestinal nutrition solution, 20 ml/Kg , UVA skin irradiation (long wave)), the dose is 10 J/cm2, each group is continuously administered for 3 days, the body weight of the mice before and after the test is measured, the content of IgG, IgA and IgM in the mouse serum is measured, and the mouse is measured The blood flow around the mouse is measured by the metric system. The content of CD19 of blood B lymphocytes and CD3 of T lymphocytes was analyzed with an automatic hematology analyzer.

　　Result: KM mice of the cyclophosphamide group and the hydrocortisone group of the hypoimmune model were established, but the 60% ethanol group and the ultraviolet irradiation group were not established in the hypoimmune model. Further experiments and research are needed to determine the weight of the mice and test the mice. The serum in the peripheral blood of mice, the levels of IgG, IgA and IgM in B and T lymphocytes, and the routine blood analysis of mice can be used as reference detection indicators to establish a model of KM mice with low immune function.

　　Conclusion: A low-immunity KM mouse animal model was established through continuous 3d oral gastrointestinal feeding of 100 mg/Kg cyclophosphamide. In terms of various testing indicators, body weight, serum immunoglobulin content and the simplicity of blood routines, these three indicators are: it is simple, easy to obtain, low testing cost, short duration, and high testing efficiency, so it can be used as a daily routine The indicators for establishing and confirming animal models in the detection operation.