动物造模,慢性炎性痛模型大鼠 z-bio 2021-12-23 748

　　OBJECTIVE: To dynamically observe the changes of pain mood in rats with chronic inflammatory pain induced by Freund's complete adjuvant (CFA), and to explore the time lag that induces anxiety and depression in rats with chronic inflammatory pain.

　　Method: All experimental rats were randomly divided into a blank control group and a model control group, and CFA was injected into the sole of the model control group to establish a chronic inflammatory pain model. Dynamic observation of changes in the mechanical withdrawal threshold (PWT) of each group of rats on days 1, 3, 7, 18, and 26 before and after modeling; 28-day open field experiment (OF), 29-day elevated O maze experiment (EZM) ) Observation)), 30d new smoke hood suppression test (NSF) after modeling, 12, 20 and 31d marble burial test (MBT) after modeling, 17, 25 and 35d after modeling, sugar water preference test (SPT) ) Rats with changes in behavior).

　　Result: One day after modeling, the PWT of the model control group was significantly reduced (P0.05). Only 31 days after MBT, compared with the blank control group, the number of buried marbles in the model control group increased significantly.

　　Conclusion: CFA-induced chronic inflammatory pain model rats can show various animal behavior changes related to anxiety-like emotions after 4 weeks, but no animal behavior changes related to depression-like emotions are observed within 5 weeks.