动物造模,酒精性心肌病小鼠模型 z-bio 2021-10-11 376

　　Objective: To establish a mouse model of alcoholic cardiomyopathy by feeding a liquid diet, and to provide an excellent animal model for studying the mechanism of alcoholic cardiomyopathy.

　　Method: 16-week-old C57 mice were fed with an alcoholic liquid diet and a non-alcoholic controlled diet (alcohol group n = 21; alcohol-free group n = 9). The feed is as follows: feeding for a total of 8 weeks, weekly weight measurement, mortality monitoring, and echocardiography and pathological examination after 8 weeks gradually increase from 4.8% to 5.4%.

　　Result: The alcohol intake group began to lose weight from the second week and continued until the eighth week, and the weight was significantly reduced compared with the control group. At the same time, 7 mice in the alcohol group died. During the alcohol intake, the control group did not die (accounting for 33.3%); echocardiography results showed that the front and back walls of the ventricle in the alcohol group were significantly thinner than the control group, but the ventricular diameter increased, and the cardiac ejection fraction and short axis shortening rate were significantly reduced. The alcohol group is smaller. Compared with the control group, the heart/body mass index of the mice increased significantly, and the HE staining results also showed that the heart of the alcohol group mice increased significantly.

　　Conclusion: After 8 weeks of alcohol solution, mouse hearts showed typical pathological features of alcoholic cardiomyopathy, including overall hypertrophy, thinning of the left ventricular wall and decreased cardiac function. Therefore, this method can successfully prepare a mouse model of alcoholic cardiomyopathy.