动物造模,过敏性结膜炎小鼠模型 z-bio 2021-10-11 791

　　Objective: To establish a mouse model of allergic conjunctivitis with different ages, different antigens, different excitatory pathways and conditions, compare the immune response status, use mice as the best model of allergic conjunctivitis, and screen crop conditions. Establish and provide new ideas and methods.

　　Methods: 65 SPF Balb/c mice, different antigens (ovalbumin, ragweed pollen, house dust mites), different excitatory routes (gavage, spray, subcutaneous injection), different mouse ages (1 week) , 2 weeks), 3 weeks old and 8 weeks old) to establish a mouse animal model of allergic conjunctivitis. T-qPCR was used to detect the relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctival tissues, and flow cytometry was used to detect the IL of splenic single cell suspension to CD4 + T cells. The ratio was -17. Compared with the negative control group, the expression rates of IL-17, CCL5 mRNA and IL-17 mRNA in the egg white albumin group, house dust mite group and ragweed pollen group all increased, and IL-17, CCL5 in the ragweed pollen group The expression of mRNA and IL-17 mRNA both increased. Compared with the negative control group, the expression ratio of IL-17 and IL-17 mRNA in the subcutaneous injection group, gavage group, spray inhalation group and the positive control group increased, and the expression of CCL5 mRNA in the gavage group was similar. Negative control group. The expression level of this group was close to that of the positive control group. The percentages of IL-17, CCL5 mRNA and IL-17 mRNA expression levels in 1-week and 2-week-old mice were similar, but significantly lower than 3- and 8-week-old mice.

　　Conclusion: Ragweed pollen has better allergenicity in establishing mouse animal models of allergic conjunctivitis. Subcutaneous injection of stimulating antigen can obtain satisfactory results in the structure of a mouse animal model of allergic conjunctivitis. There are no obvious eye symptoms or signs, but the whole body and local areas are sensitive. The immune response of 3-week-old Balb/c mice is similar to that of adult Balb/c mice.