动物造模,冠心病 z-bio 2021-04-20 293

　　Objective: To analyze the relationship between coronary heart disease deficiency and congestive syndrome with tongue symptoms, prostaglandin and thromboxane A2, pathology, etiology, and relationship between coronary heart disease in a rat model with coronary heart disease, and to explore the relationship between it and the coronary heart disease model.

　　Methods: 32 rats were randomly divided into 4 groups: K group: normal diet and water (n = 8); L group: normal feeding for 21 days and left anterior descending artery ligation (n =) 8); Y group: 21 days Food management comprehensive fatigue exercise (n = 8); Group F: 21 days food management comprehensive fatigue exercise + left anterior descending artery ligation (n = 8). After modeling, Photoshop 6.0 was used to analyze the RGB value of the tongue surface, and the RGB data distribution range table was used to evaluate the tongue color of each model. HE staining detects filamentous papillary epithelial layer, corneal layer, lamina propria height and lamina propria height. The number of capillaries in the lamina propria; immunohistochemical analysis of the positive expression of PGI2 and TXA2 and calculation of the T/P ratio.

　　Result: (1) The quality of the tongue of the K group rats was attributed to the "bright red tongue". The R value of the tongue surface of the rats in the L group was significantly lower than that of the K group due to the "darkness". "Red tongue"; The R value of the tongue surface of the rats in the Y group was significantly lower than that of the K group belonging to the "white tongue"; the R, G and B values of the tongue surface of the rats in the F group were significantly lower than the L in the group K, which belonged to "Purple tongue".

　　(2) The height of each layer of the filiform papillae of the tongue and face of the rats in the L group and the number of microvessels were not different from those in the K group. In the Y and K groups, the height of the filiform papillary keratinization layer was significantly reduced; in the F group, the filiform papillary epithelial layer and the height of the keratinization layer were significantly reduced. The microvessels of the K and L groups were significantly higher than those of the K and L groups; there was no difference in the height of the proprioceptive layer of the filamentous papilla;

　　(3) Compared with group K, the TXA2 level and T/P ratio of group L were significantly higher than that of group K; the TXA2 level and T/P ratio of group F were significantly higher than that of group K. Conclusion The RGB color characteristics of the tongue color of the Qi-deficiency syndrome and hyperemia syndrome of coronary heart disease are related to the decrease in the height of the filiform papillary epithelium and stratum corneum, and the increase in the number of capillaries in the lamina propria. Its molecular biological mechanism may be related to the imbalance of TXA2/PGI2. The comprehensive model using pathological etiology is more in line with the characteristics of the coronary heart disease model, that is, deficiency and congestion syndrome.