动物造模,非酒精性脂肪肝病食蟹猴模型 z-bio 2021-12-24 359

　　Objective: To establish a non-alcoholic fatty liver cynomolgus monkey model induced by high-fat feed, analyze the effect of feeding time of high-fat feed on the formation of animal NAFLD, and determine the feeding time of high-fat feed. Study serum biochemical indicators, as well as the formation of liver steatosis and fibrosis.

　　Method: Before the experiment, 700 healthy cynomolgus monkeys were drawn from the basal blood and fed a high-fat diet. This was part of the experiment when they were reared for 1, 2, 3, 4, 5, 6, and 7 years. Monkeys were randomly selected, anesthetized with ketamine hydrochloride, and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG) and total cholesterol (TC) were drawn from the animal’s blood. ), high-density lipoprotein (HDL), low-density lipoprotein (LDL) indicators and liver biopsy.

　　Result: After feeding on a high-fat diet for 2 years, the serum ALT, AST, TG, TC and LDL levels of cynomolgus monkeys increased significantly, while the HDL levels decreased significantly (P\u003c0.01). High-fat diet for more than 2 years was significantly correlated with ALT, AST, TG, TC, lipidation and fibrosis at the level of 0.01 (bilateral) (r is 0.127, 0.121, 0.246, 0.128, 0.306, respectively, 0.220). ), negatively correlated with HDL (r = 0.298, P\u003c0.05), and negatively correlated with LDL at the level of 0.05 (significantly correlated on both sides (r = -0.081). As time goes by, Liver, balloon degeneration, fatty liver and subsequent hepatitis and fibrosis

　　Conclusion: A high-fat diet was found in cynomolgus monkeys. Fatty liver can successfully induce the cynomolgus monkey model and promote the NAFLD process, which can significantly increase blood lipids and liver enzyme activity indicators, liver lipid accumulation and obvious inflammation infiltration .