动物造模,兔心肌梗死模型 z-bio 2021-11-26 281

　　Objective: To explore the application of cardiac catheterization technique in coronary artery occlusion to establish a rabbit myocardial infarction model.

　　Method: After general anesthesia, 25 rabbits were separated from the carotid artery, the carotid artery was punctured, and the 5F vascular sheath was inserted and fixed. The guide catheter is transported from the vascular sheath to the bottom of the sinus at the bottom of the aorta, and the microcatheter is fed into the gelfoam particle embolization through the micro-guide wire, and then delivered to the center or the distal end of the left anterior descending branch. Drugs or coils are injected into the left anterior descending artery along the microcatheter to block the left anterior descending artery to create an MI model. One month later, the heart was removed and a pathological examination was performed.

　　Result: 25 rabbits, 19 rabbits in total, were successfully delivered to the left anterior descending artery of the micro-guide wire and micro-catheter. Among the 10 rabbits in the Spring Coil group, MI successfully produced 7 rabbits and 2 died in captivity. Among the 9 rabbits in the gelatin sponge particle embolization treatment group, 7 successfully developed MI, and 1 rabbit died during feeding. There was no significant difference in the success rate of rabbit myocardial infarction and the area of rabbit myocardial infarction between the two groups.

　　Conclusion: Myocardial infarction model can be established by cardiac catheterization, and conforms to the pathological characteristics of clinical myocardial infarction, such as high success rate, stable characteristics of myocardial infarction and avoidance of thoracotomy injury.