动物造模,大鼠肥胖模型 z-bio 2021-04-20 487

　　Objective To observe the effect of using high-calorie feed to establish an obesity model in rats, as well as the differences in modeling between two species of rats, and to compare different obesity evaluation methods and obesity-sensitive animal screening methods, so as to provide animal models for studying the functions of weight-loss products reference.

　　Method 120 SD and Wistar male rats were selected, and they were randomly divided into 2 groups (blank control group and model group) according to their body weight, with 60 rats in each group. The blank control group was given a maintenance feed, and the model group was given an improved formula of high-calorie feed. The body weight and body length of the rats were recorded once a week, and the feed quality was weighed twice a week. Lee's index, food intake and food utilization were calculated. 6 weeks. After 2 or 6 weeks of feeding, 30 rats in each group were selected to detect blood biochemical indicators. After anatomy, the fat around the kidney and the fat around the testicles were taken and weighed to calculate the fat/body ratio. Analyze the correlation between the increase in body mass, Lee's index and obesity and obesity sensitivity in rats, and compare the increase in body mass and obesity of rats after feeding for 2 weeks after excluding the increase in body mass and ranking the bottom 1/3, 10%, and 20% of the rats. Correlation.

　　Results Compared with the blank control group, the body mass, Lee's index, body mass increase, food utilization rate, body fat mass and fat/body ratio of the two strains of the model group increased, food intake decreased, serum glucose and triacylglycerol The level increased, and the level of high-density lipoprotein cholesterol decreased (all P<0.05). comparing="" sd="" rats="" and="" wistar="" there="" was="" no="" significant="" difference="" in="" the="" indicators="" of="" model="" group="" p="">0.05), while the body length, Lee's index and food utilization rate of the blank control group were occasionally different (all P<0.05). The serum high-density lipoprotein cholesterol levels of rats in the blank control group were significantly different at 2 and 6 weeks of feeding (P<0.05). The correlation between the increase in body mass of the two species of rats and obesity and obesity sensitivity was significantly greater than the correlation between Lee's index and obesity and obesity sensitivity (both P<0.05). Excluding the increase in body mass, the 10% and 20% were the largest The correlation between weight gain and obesity in rats was significantly better than that of excluding the rats whose weight gain was in the bottom 1/3, and the correlation coefficient of excluding 10% was the highest.

　　Conclusion The improved high-calorie diet can successfully establish the obesity model of SD and Wistar rats, and there is no significant difference between the two species; the increase in body mass is better than Lee's index as an indicator of obesity evaluation and selection of obesity-sensitive animals, and the selection of obese-sensitive animals The method of removing the bottom 10% of the increase in body mass can be used.