动物造模,大脑中动脉闭塞模型 z-bio 2021-04-21 667

　　The monkey middle cerebral artery occlusion model is widely used in drug development, brain protection and neurological disease research. There are many manufacturing methods, including vascular compression, transorbital surgery, cerebrovascular embolization, thread embolization, photochemical methods and craniotomy. Due to the limitation of success rate, professional skills and experimental conditions.

　　Currently, the most widely used method is through orbital surgery. That is, after removing the eyeball, the posterior wall of the orbit is polished to expose the middle cerebral artery. The advantages are: 1. The method is very simple, allowing you to quickly find and ligate the main artery in the middle of the brain 2. The operation time is short, only 3. The requirements for the professional skills of the microsurgeon are relatively low. However, its shortcomings are also very obvious: 1. This operation is extremely destructive, animal welfare is also very poor, 2. The mortality rate is high, and there are many complications. 3. Complete loss of vision on one side will have a significant impact on subsequent animal testing. Therefore, we need a better way to replace it. Recently, craniotomy methods have been reported at home and abroad, including the establishment of monkey MCAO models through frontotemporal and transorbital craniotomy. Compared with transorbital surgery, these methods significantly improve animal welfare and mortality. In addition, with the deepening and diversification of scientific research, in addition to the classic MCAO model, there is an increasing need to retain or seal the selective middle cerebral artery occlusion model, the deep perforation branch of the middle cerebral artery. Blocking only one or two branches of the middle cerebral artery (cerebral artery), the middle forehead, the temporal or a part of the temporal, can cause severe paralysis of the upper limbs and avoid severe hemiplegia. The previously reported method is mainly to block the start of the middle cerebral artery, resulting in obvious hemiplegia. There is no report on the method of selectively occluding the middle cerebral artery.

　　In short, Guangdong Landao Biotechnology Co., Ltd. is studying the applied anatomy of the middle cerebral artery, and on this basis, using micro-neurosurgical technology to micro-infiltrate the middle cerebral artery and the middle cerebral artery. Use the middle cerebral artery of the pterygoid approach under the microscope. Selectively block branches. The results showed that monkeys have unique characteristics in the shape, pathways and bifurcations of the middle cerebral artery, and have larger collateral circulation than humans. Our method can not only create classic MCAO models, but also selectively block specific branches of the middle cerebral artery to meet various experimental designs and requirements. The surgical success rate is high, the mortality rate is low, the additional damage is small, the animal welfare is good, and it can be mass-produced.