动物造模,微小骨粒回植模型 z-bio 2021-10-07 391

　　Objective: To establish a rabbit model of bone replantation after opening the window of the lumbar vertebral lamina in rabbits, and to provide clinical experimental evidence.

　　Method: Divide 18 New Zealand male rabbits into two groups. In the control group, after general anesthesia, the L5 spinous process was placed in the center, the left L5 lamina was completely exposed, and the lamina and ligamentum flavum were slowly bitten with micro gun forceps, and fenestration was performed. Open the bone window and expose it to approximately 0.8 light. Dimensions x 0.3 cm. Immediately after stitching. After opening the left window of L5, the experimental group cut out a piece of bone bitten by the window, put it into a medical collagen sponge to form an arched bridge, put it into the epidural window and sew it. After the operation, observe the CT and histological changes of the opening position of the lamina.

　　Results: According to the results of CT examination, the small bone particles of the experimental group formed a thin bone plate at the opening of the window 8 weeks after the operation. The bone plate formed after 12 weeks was thick, the trabecula was continuous, and the shape and volume of the spinal cord did not change significantly. The spinal cord is compressed. The control group had a small amount of bone hyperplasia at 8 weeks and 12 weeks after the window was opened, and the surrounding adhesion tissue infiltrated the spinal canal without rebuilding the spinal canal.

　　Conclusion: By re-implanting small autologous bone particles into the opening of the rabbit lamina for bone reconstruction, it can prevent scars from invading the spinal canal and reduce spinal adhesions.